วันพฤหัสบดีที่ 29 มกราคม พ.ศ. 2558

A NEW FAMILY OF BIOORTHOGONALLY APPLICABLE FLUOROGENIC LABELS
Abstract: Synthetic procedures for the construction of fluorogenic azido-labels were developed. Photophysical properties were elaborated by experimental and theoretical investigations. Of the newly synthesized fluorogenic and bioorthogonally applicable dyes two were selected on the basis of their fluorogenic performance and further subjected to in vitro and in vivo studies. Both tags exhibited excellent fluorogenic properties as in aqueous medium, the azide form of the selected dyes is virtually non-fluorescent, while their “clicked” triazole congeners showed intense fluorescence. One of these labels showed a very large Stokes shift. To the best of our knowledge this is the first reported case of mega-Stokes type of fluorogenic labels. These studies have justified that these two fluorogenic tags are remarkably suitable for bioorthogonal tagging schemes. The developed synthetic approach together with the theoretical screen of possible fluorogenic tags will enable the generation of libraries of such tags in the future.

Reference
Herner, A.; Nikić, I.; Kállay, M.; Lemke, E. A.; Kele, P. Org. Biomol. Chem. 2013, 11, 3297–3306.

Results/Finding
The azide-alkyne groups in click chemistry are suitable methodology for bioorthogonal tagging schemes.

Citation
1) Herner et al. (2013) reported that the bioorthogonally applicable dyes were labeled via click chemistry in aqueous medium condition (p. 3297).


2) Herner et al. (2013) found that the azide form exhibited non-fluorescent properties while the triazole-linkage form revealed excellent fluorescent (p. 3297).

5 ความคิดเห็น:

  1. Hi Boonsongd

    I have a comment on your reference in APA-style. it should be.....
    Herner, A., Nikić, I., Kállay, M., Lemke, E., & Kele, P. (2013). A new family of bioorthogonally applicable fluorogenic labels. Org. Biomol. Chem., 11(20), 3297.

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  2. Glad this is working now! :D

    et al. does not need to be italicized.

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  3. An excellent fluorogenic substrate for measuring the peptidylglutamyl-peptide-hydrolyzing (PGPH) activity of the 20S proteasome, which is excited at 340-360 nm and emits at 440-460 nm. It is typically used in cell lysates after experimental treatment. Z-Leu-Leu-Leu-AMC

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