A NEW FAMILY OF
BIOORTHOGONALLY APPLICABLE FLUOROGENIC LABELS
Abstract: Synthetic
procedures for the construction of fluorogenic azido-labels were developed.
Photophysical properties were elaborated by experimental and theoretical
investigations. Of the newly synthesized fluorogenic and bioorthogonally
applicable dyes two were selected on the basis of their fluorogenic performance
and further subjected to in vitro and in vivo studies. Both tags exhibited
excellent fluorogenic properties as in aqueous medium, the azide form of the
selected dyes is virtually non-fluorescent, while their “clicked” triazole
congeners showed intense fluorescence. One of these labels showed a very large Stokes
shift. To the best of our knowledge this is the first reported case of
mega-Stokes type of fluorogenic labels. These studies have justified that these
two fluorogenic tags are remarkably suitable for bioorthogonal tagging schemes.
The developed synthetic approach together with the theoretical screen of
possible fluorogenic tags will enable the generation of libraries of such tags
in the future.
Reference
Herner,
A.; Nikić, I.; Kállay, M.; Lemke, E. A.; Kele, P. Org. Biomol.
Chem. 2013, 11, 3297–3306.
Results/Finding
The
azide-alkyne groups in click chemistry are suitable methodology for
bioorthogonal tagging schemes.
Citation
1) Herner et
al. (2013) reported that the bioorthogonally applicable dyes were labeled
via click chemistry in aqueous medium condition (p. 3297).
2) Herner et
al. (2013) found that the azide form exhibited non-fluorescent properties
while the triazole-linkage form revealed excellent fluorescent (p. 3297).
Hi Boonsongd
ตอบลบI have a comment on your reference in APA-style. it should be.....
Herner, A., Nikić, I., Kállay, M., Lemke, E., & Kele, P. (2013). A new family of bioorthogonally applicable fluorogenic labels. Org. Biomol. Chem., 11(20), 3297.
thank you ka
ตอบลบthank you ka
ตอบลบGlad this is working now! :D
ตอบลบet al. does not need to be italicized.
An excellent fluorogenic substrate for measuring the peptidylglutamyl-peptide-hydrolyzing (PGPH) activity of the 20S proteasome, which is excited at 340-360 nm and emits at 440-460 nm. It is typically used in cell lysates after experimental treatment. Z-Leu-Leu-Leu-AMC
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